TERRA-REF combines advanced sensing with novel analytical approaches to accelerate breeding
The Transportation Energy Resources from Renewable Agriculture Phenotyping Reference Platform (TERRA-REF) aims to transform plant breeding by using remote sensing to quantify plant traits such as plant architecture, carbon uptake, tissue chemistry, water use, and other features to predict the yield potential and stress resistance of 400+ diverse sorghum lines.
TERRA-REF is conducting high-throughput phenotyping to connect discoveries to field performance
Breeding is currently limited by the speed at which phenotypes can be measured, and the information that can be extracted from these measurements. Current instruments used to quantify plant traits do not scale to the thousands or tens of thousands of individual plants that need to be evaluated in a breeding program. The TERRA-REF field scanner system scans over 1 acre of plants, collecting thousands of daily measurements throughout the growing season that are used to determine plant phenotypes and inform breeding decisions.
The field level phenotypic data combined with the genomic data is helping us to identify the differences between each line and the reference genome sequence for sorghum. We are using bioinformatics and quantitative genetics to characterize the observed genetic variation and identify genomic regions controlling biomass, plant architecture, and photosynthetic traits.
TERRA-REF is using large-scale genome sequencing to drive phenotype-genotype associations and gene discovery
There is enormous potential for sorghum crop improvement. There are 50,000 sorghum accessions in the U.S. germplasm collection and most are unused and unstudied. TERRA-REF is analyzing a sorghum bioenergy association panel (BAP) that includes diverse sweet and biomass lines from all five sorghum races. The BAP captures geographic, racial, and genomic diversity.
TERRA-REF has already sequenced 384 of the lines with an average sequence coverage of 20x per line. Genome-wide association studies (GWAS) are now underway.
TERRA-REF is providing reference quality data as a community resources
TERRA-REF is developing a data storage and computing system that provides researchers with access to all of the ‘raw’ data and derived plant phenotypes (traits). Data from sensors at a variety of locations across the US will be transferred to one location.
The reference data will facilitate data sharing and re-use of data by providing metadata, provenance for derived data sets, and standardized data processing workflows. It will include geospatial infrastructure for efficiently querying and transforming key datasets and tools that enable researchers to access, archive, use, and contribute data products.
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